Solvent Findings > Amusement

Possible Amusement Gene Sequences

Aperio favors this sequence

 KOOORGGGGGGROOORGGGGGGGROGGGGGK

While it's not conclusive, RGGGGGG has duped and we've seen GGGGGGR 3x and we've not seen a RGGGGGR yet.

Ariella Solvent Data

RGGGGGG	KOOORGG	OGGGGGK	OORGGGG	GGGGGGR	RGGGGGG	OOORGGG
GGGGGRO	GGGGROG	GGGGGGG	GGGROGG	GROGGGG

 KOOORGG
  OOORGGG
   OORGGGG
    ORGGGGG
     RGGGGGG
      GGGGGGR
       GGGGGRO
        missing
         missing
          GGROOO
           missing
            ROOORG
             OOORGGG
              OORGGGG
                RGGGGGG
                 GGGGGGG
                  GGGGGGR
                   GGGGGRO
                    GGGGROG
                     GGGROGG
                      missing
                       GROGGGG
                         OGGGGGK
 KOOORGGGGGGROOORGGGGGGGROGGGGGK

Made two crosses of Amusement8G/Appreciation2Y KU1 to put a Y in the middle of the Gs on Amusement. Solvent data for that cross below.

GGGGGG

OOORGG

GGGGRO

GGROOY

KU1 should hit 41.67% to 44.12% on a genome, this time a Y. Looks like GGROOY used to be GGROOO. On a 29 length genome the Y (or third O) would hit on the 12th/13th gene which would put 5 Gs between KOOOR and ROOOR.

 KOOORGGGGGGGROGGGGGK  Note: solvents below show different, elongated genome now.

Made two crosses putting a Y in gene 7 and 18 of Amusement, in the middle of the two long G sequences. Solvent data for that cross below. KOOORGGGyGGROOORGGGyGGGROGGGGGK - assumption

OORGGG - 4	GGGGGK	ORGGGY	GGGGRO - 2	OOORGG	ROOORG	GGROGG
RGGGYG	GYGGGG	RGGGYGG - 2	GGYGGGG - 2	GYGGGGR - 2

Aperio Solvent Data

 OOORGGG
 GGGGGGR x2
 OGGGGGK
 ROGGGGG
 GGGGGRO x2
 ROOORG

Aperio Solvent Data (200 Milky trials)

 (45.5%) (91) GGG 1111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111
 (04.0%) (08) GGK 11111111
 (06.0%) (12) GGR 111111111111
 (06.5%) (13) GRO 1111111111111
 (03.5%) (07) KOO 1111111
 (02.5%) (05) OGG 11111
 (06.5%) (13) OOO 1111111111111
 (06.0%) (12) OOR 111111111111
 (08.0%) (16) ORG 1111111111111111
 (06.5%) (13) RGG 1111111111111
 (02.5%) (05) ROG 11111
 (02.5%) (05) ROO 11111

Analysis (independent of the more complex solvents to validate the technique)

• Assuming +/- 2.5% variance, all the non-GGG combinations fall within the same range with the marginal exception of ORG. Since both endpoints (which by definition will have 1 occurrence) are at the upper end of this range, I'm willing to expand the range to include ORG because we know the random number generation is weak and it's non-sensical to create a new bands of 2 occurrences to accommodate ORG when the endpoints would be present in that band.
• GGG appears 9x as often as any other sequence, which suggests 11 Gs in a row OR two strings of Gs.
• GGK terminates one of the GGG strings. GGR terminates another. It is possible that one or both could just be a double, but then we'd have to have repetitions to account for the GGG strings separately.
• OGG begins a GGG string. RGG starts another. Two strings of GGG(x) and GGG(y) are conclusive. I'm using (x), (y), and (z) to indicate repetitions of the sequence. In some single gene sequences, I'm referring to the repetitions of the same letter in the gene to give its length and I need to adopt a clearer notations like [z] - Aperio

 KOO 1
  OOO 1
   OOR 1
    ORG 1
     RGG 1

       GGG(x) 

          GGR 1
           GRO 1
            ROG 1
             OGG 1

               GGG(y)

                   GGK

Combined with more complex clues

• RGG starts no less than 6 Gs. This accounts for at least 4 of out repetitions.
• OGG stats no more than 6 Gs because it's terminated by a K. This accounts for 3 of our 9 repetitions.
• We have a GGGGGGG[7] clue, so we know that RGG must lead to at least 5 of our repetitions.
• Since we have no expectation of a 3rd string of GGG(z), we must assume that RGG starts a sequence of 8 Gs to permit 6 repetitions.
• [POST-MORTEM] I have missed ROO in this analysis.

 KOOORGGGGGGGGROGGGGGK
 KOO 1
  OOO 1
   OOR 1
    ORG 1
     RGG 1
 KOOORGG CONFIRMED
      GGGGGGGG GGG(x=8)
  OOORGGG CONFIRMED
   OORGGGG CONFIRMED
     RGGGGGG CONFIRMED
      GGGGGGG LIKELY
       GGGGGGG LIKELY(2)
            GGR 1
        GGGGGGR CONFIRMED
             GRO 1
         GGGGGRO CONFIRMED
              ROG 1
          GGGGROG CONFIRMED
               OGG 1
           GGGROGG CONFIRMED
                GGGGG GGG(y=5)
             GROGGGG CONFIRMED
               OGGGGGK CONFIRMED
                   GGK
 KOOORGGGGGGGGROGGGGGK

• This is really tight, but ROOORG from my later Crystal trial is UNACCOUNTED FOR - Aperio
• [POST-MORTEM] I still have missed ROO in this analysis.
• This suggests another string of OOO to account for ROOORG.
• OOR density supports this string. ROO density does not. ORG density DEFINITELY supports this but was overlooked intentionally.
• This implies another RGG string which is supported, also.
• If 8% is the 2nd band, then 44% is 11 repeats of GGG and 28% is 12 repeats
• if 7.5% is the 2nd band, then 45% is 12 repeats. I lean towards 12 here.

 11-12 GGG 1111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111
 1 GGK 11111111
 2 GGR 111111111111
 2 GRO 1111111111111
 1 KOO 1111111
 1 OGG 11111
 2 OOO 1111111111111
 2 OOR 111111111111
 2 ORG 1111111111111111
 2 RGG 1111111111111
 1 ROG 11111
 1 ROO 11111

 KOOORGGGGGGROOORGGGGGGGROGGGGGK
 KOOORGG
 KOO 1
  OOO 1
   OOR 1
    ORG 1
     RGG 1
 KOOORGGGGGGROOORGGGGGGGROGGGGGK
   OORGGGG
 KOOORGGGGGGROOORGGGGGGGROGGGGGK
     RGGGGGG
      GGG 1
       GGG 2
        GGG 3
         GGG 4
 KOOORGGGGGGROOORGGGGGGGROGGGGGK
      GGGGGGR
          GGR 1
 KOOORGGGGGGROOORGGGGGGGROGGGGGK
       GGGGGRO
           GRO 1
 KOOORGGGGGGROOORGGGGGGGROGGGGGK
            ROOORG
            ROO 1
             OOO 2
              OOR 2
               ORG 2
 KOOORGGGGGGROOORGGGGGGGROGGGGGK
              OORGGGG
                RGG 2
               ???????
 KOOORGGGGGGROOORGGGGGGGROGGGGGK
                RGGGGGG
                 GGG 5
                  GGG 6
                   GGG 7
                    GGG 8
 KOOORGGGGGGROOORGGGGGGGROGGGGGK
                 GGGGGGG (GGGx5)
                     GGG 9
 KOOORGGGGGGROOORGGGGGGGROGGGGGK
                  GGGGGGR
                      GGR 2
 KOOORGGGGGGROOORGGGGGGGROGGGGGK
                   GGGGGRO
                       GRO 2
 KOOORGGGGGGROOORGGGGGGGROGGGGGK
                    GGGGROG
                        ROG 1
 KOOORGGGGGGROOORGGGGGGGROGGGGGK
                     GGGROGG
                         OGG 1
 KOOORGGGGGGROOORGGGGGGGROGGGGGK
                       GROGGGG
                          GGG 10
                           GGG 11
                        ???????
 KOOORGGGGGGROOORGGGGGGGROGGGGGK
                         OGGGGGK (GGGx3)
                            GGG 12
                             GGK 1
 KOOORGGGGGGROOORGGGGGGGROGGGGGK